CD4+ T cells were separated from dLNs by magnetic-activated cell sorting (MACS) separation (Miltenyi Biotec, Bergisch Gladbach, Germany). 2.16. T cells that induce the expression IGLC1 of CD40L and CD25 (IL-2 receptor) for B cell helpers and proliferation [20,21]. The NFB pathway, including p65 translocation and MAPK pathway, are known to be involved in T … Continue reading CD4+ T cells were separated from dLNs by magnetic-activated cell sorting (MACS) separation (Miltenyi Biotec, Bergisch Gladbach, Germany)